LOCUS       UGT2A1-pGEc2            6696 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(6174..6303)
                     /note="3'-ITR"
     misc_feature    complement(5872..6086)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    5324..5865
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4503..4525
                     /note="attB2"
     misc_feature    5303..5308
                     /note="BstB1 site"
     misc_feature    4526..5221
                     /note="Super GFP"
     misc_feature    5222..5272
                     /note="AviTag"
     misc_feature    5273..5296
                     /note="His Tag"
     misc_feature    3011..4480
                     /note="OPTIMIZED UGT2A1 coding region"
     PCR_frame       3011..4502
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    4481..4501
                     /note="TEV"
     vector          join(4511..6696,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..4510
                     /source="UGT2A1 pDONR"
                     /type="Entry Clone"
     source          1..6696
                     /dnas_title="UGT2A1-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGCTGAACA ACCTGCTGCT GTTCAGCCTG CAGATCTCCC TGATCGGCAC
     3061 AACACTGGGC GGCAATGTGC TGATCTGGCC TATGGAAGGC TCCCACTGGC TGAACGTGAA
     3121 GATCATCATC GACGAGCTGA TCAAGAAAGA ACACAACGTG ACCGTGCTGG TGGCCTCTGG
     3181 CGCCCTGTTT ATCACCCCTA CAAGCAACCC TAGCCTGACC TTCGAGATCT ACAAGGTGCC
     3241 CTTCGGCAAA GAACGGATCG AGGGCGTGAT CAAGGACTTC GTGCTGACAT GGCTGGAAAA
     3301 CAGACCCAGC CCCAGCACCA TCTGGCGGTT TTACCAGGAA ATGGCCAAAG TCATCAAGGA
     3361 TTTCCACATG GTGTCTCAGG AAATCTGCGA CGGCGTCCTG AAGAACCAGC AGCTGATGGC
     3421 CAAGCTGAAG AAAAGCAAGT TCGAGGTCCT GGTGTCCGAC CCTGTGTTCC CTTGTGGCGA
     3481 TATTGTGGCC CTGAAGCTGG GCATCCCCTT CATGTACAGC CTGAGATTCA GCCCCGCCAG
     3541 CACCGTGGAA AAGCACTGTG GCAAAGTGCC CTACCCTCCT AGCTATGTGC CTGCCGTGCT
     3601 GAGCGAGCTG ACCGATCAGA TGAGCTTCAC CGACCGGATC AGAAACTTCA TTAGCTACCA
     3661 TCTCCAGGAC TACATGTTCG AGACTCTGTG GAAGTCCTGG GACAGCTACT ACAGCAAAGC
     3721 CCTGGGCAGA CCTACCACCC TGTGCGAGAC AATGGGAAAG GCCGAGATCT GGCTGATCAG
     3781 AACCTACTGG GACTTCGAGT TCCCCAGACC CTACCTGCCC AACTTCGAAT TTGTGGGCGG
     3841 ACTGCACTGC AAGCCTGCCA AGCCTCTGCC CAAAGAGATG GAAGAGTTCA TCCAGAGCAG
     3901 CGGCAAGAAT GGCGTGGTGG TGTTTAGCCT GGGCTCCATG GTCAAGAACC TGACCGAAGA
     3961 GAAGGCCAAC CTGATCGCTT CTGCCCTGGC CCAGATTCCT CAGAAAGTGC TGTGGCGGTA
     4021 CAAGGGCAAG AAGCCAGCCA CCCTGGGAAA CAACACCCAG CTGTTCGACT GGATCCCCCA
     4081 GAATGATCTG CTGGGCCACC CTAAGACCAA GGCCTTTATC ACACACGGCG GCACCAATGG
     4141 CATCTACGAG GCCATCTATC ACGGCGTGCC AATGGTCGGA GTGCCTATGT TTGCCGACCA
     4201 GCCCGACAAC ATTGCCCATA TGAAGGCTAA GGGCGCTGCC GTGGAAGTGA ACCTGAATAC
     4261 CATGACCAGC GTGGACCTGC TGAGTGCCCT GAGAACAGTG ATCAACGAGC CCAGCTACAA
     4321 AGAAAACGCC ATGCGGCTGA GCCGGATCCA CCACGATCAG CCTGTGAAAC CTCTGGACAG
     4381 AGCCGTGTTC TGGATCGAGT TCGTGATGAG ACACAAGGGG GCCAAGCACC TGAGAGTGGC
     4441 CGCTCATGAT CTGACCTGGT TCCAGTACCA CAGCCTGGAC gaaaacttgt actttcaagg
     4501 ctCCAGCTTT CTTGTACAAA GTGGTATGAG CAAGGGCGAG GAACTGTTCA CCGGCGTGGT
     4561 GCCCATCCTG GTGGAACTGG ATGGCGACGT GAACGGCCAC AAGTTCTCTG TGCGGGGAGA
     4621 GGGCGAAGGC GACGCCACAA ATGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA
     4681 GCTGCCCGTG CCTTGGCCTA CCCTCGTGAC CACACTGACC TACGGCGTGC AGTGCTTCAG
     4741 CAGATACCCC GACCATATGA AGCGGCACGA CTTCTTCAAG AGCGCCATGC CCGAGGGCTA
     4801 CGTGCAGGAA CGGACCATCA GCTTCAAGGA CGACGGCACC TACAAGACCA GAGCCGAAGT
     4861 GAAGTTCGAG GGCGACACCC TCGTGAACCG GATCGAGCTG AAGGGCATCG ATTTCAAAGA
     4921 GGACGGCAAC ATCCTGGGCC ACAAGCTGGA GTACAACTTC AACAGCCACA ACGTGTACAT
     4981 CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGGC ACAACGTGGA
     5041 AGATGGCAGC GTGCAGCTGG CCGACCACTA CCAGCAGAAC ACCCCCATCG GAGATGGCCC
     5101 CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGAGC GTGCTGAGCA AGGACCCCAA
     5161 CGAGAAGCGG GACCACATGG TGCTGCTGGA ATTCGTGACC GCCGCTGGCA TCACCCACGG
     5221 CATGAGCGGC CTGAACGACA TCTTCGAGGC CCAGAAAATC GAGTGGCACG AGCACCACCA
     5281 TCATCACCAT CACCACTGAT GATTCGAAAA ATAATAAGGA TCCAATCAAC CTCTGGATTA
     5341 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG
     5401 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC
     5461 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA
     5521 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC
     5581 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT
     5641 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC
     5701 CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG
     5761 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC
     5821 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGAGATC TGCCTCGACT
     5881 GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG
     5941 GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG
     6001 AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG
     6061 GAAGACAATA GCAGGCATGC TGGGGACTCG AGTTAAGGGC GAATTGCCGA TTAGGATCTT
     6121 CCTAGAGCAT GGCTACGTAG ATAAGTAGCA TGGCGGGTTA ATCATTAACT ACAAGGAACC
     6181 CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG
     6241 ACCAAAGGTC GCCCGACGCC CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG
     6301 CAGCCTTAAT TAAGAGCTCA TGGCGCGCCT AGGCCTTGAC GGCCTTCCGC CAATTCGCCC
     6361 TATAGTGAGT CGTATTACGT CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA
     6421 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG
     6481 TAATAGCGAA GAGGCCCGCA CCGAAACGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG
     6541 AATGGGAGCG CCCTGTAGCG GCCACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA
     6601 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC
     6661 GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAG
//

